Integration site analysis of viral vectors with Target Enrichment Sequencing (TES)
With the advent of next generation sequencing (NGS) technologies, new approaches are promising to identify vector IS in a more comprehensive way, qualitatively and quantitatively. In line with cancer genomics studies where all exon studies are used, we offer partial or complete vector genome target enrichment sequencing (TES).
The results of vector genome TES yield information on clonality (integration sites), quantitative individual clonal contributions and vector genome stability. The additional enrichment of cellular subgenomic DNA regions in parallel to vector sequences further allows to define the vector copy number (VCN) in the analyzed DNA samples (comparable to a seperate qPCR) .